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Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children

Identifieur interne : 00A348 ( Main/Exploration ); précédent : 00A347; suivant : 00A349

Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children

Auteurs : E. Könönen [Finlande] ; M.-L. V Is Nen [Finlande] ; S. M. Finegold [États-Unis] ; R. Heine [Finlande] ; H. Jousimies-Somer [Finlande]

Source :

Anaerobe [ 1075-9964 ] ; 1996.

RBID : ISTEX:5DF59AAF439CD927216C177F355AA70E3E6C18A2

English descriptors

Abstract

Abstract: During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmentedPrevotella-like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.

Url:

https://api.istex.fr/document/5DF59AAF439CD927216C177F355AA70E3E6C18A2/fulltext/pdf

DOI: 10.1006/anae.1996.0042

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmentedPrevotella-like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.</div>
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Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children

Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children

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English descriptors

Abstract

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